Article
A semi-automated method for analysis of intron 13 and intron 22 dinucleotide repeat polymorphisms of the factor VIII gene.
Clinical and laboratory haematology - 1 Jun 1996
Yip B, Chan V, Chan T K
Abstract excerpt
A semi-automated method has been developed for the simultaneous analysis of two micro-satellite repeat polymorphisms located in intron 13 and 22 of the Factor VIII gene. The fluorescent dyes, 6-FAM-and HEX-phosphoramidites were used to 5'-end label the respective 5' primers of these two microsate...
Topics
- Alleles
- Automation
- DNA Mutational Analysis
- DNA Primers
- Dinucleotide Repeats
- Electrophoresis, Polyacrylamide Gel
- Factor VIII
- Female
- Fluorescent Dyes
- Genotype
- Haplotypes
- Hemophilia A
- Humans
- Introns
- Leukocytes
- Male
- Polymerase Chain Reaction
- Software