Article
A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome.
Molecular microbiology - 1 Jul 1996
Redenbach M, Kieser H M, Denapaite D, Eichner A, Cullum J, Kinashi H, Hopwood D A
Abstract excerpt
A Supercos-1 library carrying chromosomal DNA of a plasmid-free derivative of Streptomyces coelicolor A3(2) was organized into an ordered encyclopaedia of overlapping clones by hybridization. The minimum set of overlapping clones representing the entire chromosome (with three short gaps) consists...
Topics
- Chromosome Mapping
- Cloning, Molecular
- Cosmids
- DNA, Bacterial
- Escherichia coli
- Gene Library
- Genes, Bacterial
- Genetic Complementation Test
- Genetic Markers
- Molecular Sequence Data
- Multigene Family
- Mutation
- Recombination, Genetic
- Repetitive Sequences, Nucleic Acid
- Streptomyces
- Transformation, Genetic