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Article

Analysis of wild-type and mutant aspartate aminotransferases using integrated rate equations.

Biochimica et biophysica acta - 13 Sept 1996

Schiller M R, Holmes L D, Boeker E A

Abstract excerpt

A general integrated rate equation was fit to reaction progress curves catalyzed by wild-type E. coli aspartate aminotransferase and the site-specific mutant enzymes, H193Q and Y70F. A nonlinear step-regression code, revised for this study selected from all kinetic constants in a general integrated rate equation for all unbranched enzyme mechanisms with stoichiometries upto two substrates and two products...

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PMID: 8841376
DOI: 10.1016/0167-4838(96)00083-0

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