Transport pathway, maturation, and targetting of the vesicular stomatitis virus glycoprotein in skeletal muscle fibers.

Abstract excerpt

We have infected isolated skeletal muscle fibers with the vesicular stomatitis virus or the mutant tsO45, whose glycoprotein is blocked in the endoplasmic reticulum at 39 degrees C. Immunofluorescence analysis for the viral glycoprotein indicated that the fibers were infected over their entire length at a virus dose of 10(9)/ml. When we infected the myofibers with the tsO45 mutant at 39 degrees C, the viral...

Topics

• Animals
• Biological Transport, Active
• Endoplasmic Reticulum
• Glycoproteins
• Golgi Apparatus
• In Vitro Techniques
• Membrane Glycoproteins
• Microscopy, Fluorescence
• Microscopy, Immunoelectron
• Muscle, Skeletal