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Replication of Hepatitis C Virus in Cultured Non‐neoplastic Human Hepatocytes

1 Aug 1996

Abstract excerpt

We established a replication system for hepatitis C virus (HCV) using the PH5CH non-neoplastic human hepatocyte line that had been immortalized with simian virus 40 large T antigen. In cells inoculated with sera derived from two HCV-positive blood donors, positive-stranded HCV RNA was detected up to 30 days postinoculation (p.i.). Semi-quantitative analysis of HCV RNA revealed that HCV multiplied during the...

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PMID: 8797883
DOI: 10.1111/j.1349-7006.1996.tb02101.x

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