Article
Demonstration and genotyping of pestivirus RNA from mammalian cell lines.
Microbiology and immunology - 1 Jan 1995
Harasawa R, Mizusawa H
Abstract excerpt
We examined 20 cell lines of various animal origins for the presence of pestivirus contamination by the reverse transcription-polymerase chain reaction (RT-PCR), and found 15 (75%) cell lines were positive. The RT-PCR products of the 5' untranslated region (UTR) of pestivirus genome were sequenced and subjected to genotyping. Stem-loop structures at three variable regions in the 5' UTR render genotyping of the...
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