Article
Cloning and targeted gene disruption of EXG1, encoding exo-beta 1, 3-glucanase, in the phytopathogenic fungus Cochliobolus carbonum.
Applied and environmental microbiology - 1 Feb 1994
Schaeffer H J, Leykam J, Walton J D
Abstract excerpt
The phytopathogenic fungus Cochliobolus carbonum produces an extracellular enzyme capable of degrading beta 1,3-glucan in an exolytic manner. On the basis of partial amino acid sequences of the purified enzyme, two degenerate oligonucleotides were synthesized and used as PCR primers to amplify a 1.1-kb fragment of corresponding genomic DNA. The PCR product was used to isolate the genomic copy of the gene, called...
Topics
- Amino Acid Sequence
- Ascomycota
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Glucan 1,3-beta-Glucosidase
- Molecular Sequence Data
- Mutation
- Transformation, Genetic
- beta-Glucosidase