Article
Identification of two apolipoprotein variants, A-I Karatsu (Tyr 100-->His) and A-I Kurume (His 162-->Gln).
Clinical genetics - 1 Feb 1996
Moriyama K, Sasaki J, Matsunaga A, Takada Y, Kagimoto M, Arakawa K
Abstract excerpt
We identified two apolipoprotein (apo) A-I variants, using isoelectric focusing gel electrophoresis: apo A-I Karatsu, which had a relative charge of +1 compared to normal apo A-I4, and apo A-I Kurume, which had a relative charge of -1. Direct sequence analysis of the PCR-amplified DNA from the pr...
Topics
- Adult
- Apolipoprotein A-I
- Base Sequence
- DNA Restriction Enzymes
- Electrophoresis, Gel, Two-Dimensional
- Female
- Genetic Markers
- Genetic Variation
- Humans
- Immunoblotting
- Isoelectric Focusing
- Lipoproteins
- Male
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length