Article
A rat genetic map constructed by representational difference analysis markers with suitability for large-scale typing.
Proceedings of the National Academy of Sciences of the United States of America - 30 Apr 1996
Toyota M, Canzian F, Ushijima T, Hosoya Y, Kuramoto T, Serikawa T, Imai K, Sugimura T, Nagao M
Abstract excerpt
Representational difference analysis (RDA) was applied to isolate chromosomal markers in the rat. Four series of RDA [restriction enzymes, BamHI and HindIII; subtraction of ACI/N (ACI) amplicon from BUF/Nac (BUF) amplicon and vice versa] yielded 131 polymorphic markers; 125 of these markers were...
Topics
- Alleles
- Animals
- Blotting, Southern
- Chromosome Mapping
- Crosses, Genetic
- DNA
- Deoxyribonuclease BamHI
- Deoxyribonuclease HindIII
- Electrophoresis, Agar Gel
- Genetic Markers
- Polymerase Chain Reaction
- Rats
- Rats, Inbred ACI
- Rats, Inbred BUF