Detection of point mutations in human tyrosinase gene by improved allele-specific amplification.

Abstract excerpt

Allele-specific amplification (ASA) is a simple and non-radioactive technique for detecting known point mutations that produce genetic diseases. Although this technique is based on the specific amplification of the target allele by a polymerase chain reaction (PCR) with allele-specific primers, t...

Topics

• Albinism, Oculocutaneous
• Alleles
• Base Sequence
• Female
• Gene Amplification
• Humans
• Male
• Molecular Sequence Data
• Monophenol Monooxygenase
• Pedigree
• Point Mutation