Article
Comparison of arbitrarily primed PCR with restriction endonuclease and immunoblot analyses for typing Clostridium difficile isolates.
Journal of clinical microbiology - 1 Dec 1995
Tang Y J, Houston S T, Gumerlock P H, Mulligan M E, Gerding D N, Johnson S, Fekety F R, Silva J
Abstract excerpt
Arbitrarily primed PCR (AP-PCR) was used to genotype 26 clinical isolates of Clostridium difficile previously analyzed by immunoblotting (IB) and 20 isolates typed by restriction endonuclease analysis (REA) with HindIII. Two levels of differentiation were achieved with the AP-PCR approach by use of two different arbitrary primers. With the 19-mer arbitrary primer T-7 (first level of differentiation), a good...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
