Article
Comparison of arbitrarily primed PCR with restriction endonuclease and immunoblot analyses for typing Clostridium difficile isolates.
Journal of clinical microbiology - 1 Dec 1995
Tang Y J, Houston S T, Gumerlock P H, Mulligan M E, Gerding D N, Johnson S, Fekety F R, Silva J
Abstract excerpt
Arbitrarily primed PCR (AP-PCR) was used to genotype 26 clinical isolates of Clostridium difficile previously analyzed by immunoblotting (IB) and 20 isolates typed by restriction endonuclease analysis (REA) with HindIII. Two levels of differentiation were achieved with the AP-PCR approach by use...
Topics
- Bacterial Typing Techniques
- Base Sequence
- Clostridioides difficile
- DNA Primers
- DNA Restriction Enzymes
- DNA, Bacterial
- Deoxyribonuclease HindIII
- Evaluation Studies as Topic
- Genotype
- Humans
- Immunoblotting
- Molecular Sequence Data
- Polymerase Chain Reaction
- Prohibitins