5‐aza deoxycytidine‐induced inhibition of differentiation of spermatogonia into spermatocytes in the mouse

Abstract excerpt

In order to explore the significance of DNA methylation in proliferation and differentiation of germ cells in testis, 5-aza,2'-deoxyCytidine (5-azaCdR), a hypomethylating agent, was administered in vivo to neonatal mice having only spermatogonial (premeiotic) cells. End-labeling of the MspI, HpaII, and HhaI digested DNA revealed considerable loss of methylation following the treatment. Cellular and histological...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Epigenetics and DNA Methylation
• Share to Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
• Share to Reproductive Biology and Fertility