Article
Biotinylation of cell surface MHC molecules: a complementary tool for the study of MHC class II polymorphism in cattle.
Journal of immunological methods - 16 Jan 1996
Schuberth H J, Kroell A, Leibold W
Abstract excerpt
Biotinylation of cell surface proteins is often used as an alternative to radioactive labelling procedures, but very little is known about the labelling of the different allelic products of polymorphic antigenic systems. In this report, we demonstrate that NHS-LC-biotin labels bovine MHC class II molecules with different efficiencies for several allelic forms of this polymorphic system compared to conventional...
Topics
- Alleles
- Animals
- Antibodies, Monoclonal
- Binding Sites, Antibody
- Biotin
- Cattle
- Cell Survival
- Genes, MHC Class II
- Histocompatibility Antigens Class II
- Leukocytes, Mononuclear
- Methionine
- Polymorphism, Genetic
- Sulfur Radioisotopes
