Article
Identification of Zucchini yellow mosaic potyvirus by RT-PCR and analysis of sequence variability.
Journal of virological methods - 1 Sept 1995
Thomson K G, Dietzgen R G, Gibbs A J, Tang Y C, Liesack W, Teakle D S, Stackebrandt E
Abstract excerpt
A reverse transcription-polymerase chain reaction (RT-PCR) method was used to identify Zucchini yellow mosaic virus (ZYMV) in leaves of infected cucurbits. Oligonucleotide primers which annealed to regions in the nuclear inclusion body (NIb) and the coat protein (CP) genes, generated a 300-bp product from ZYMV and also from the closely related watermelon mosaic virus type 2 (WMV-2). However, no product was...
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