An improved method of isolating fetal human retinal pigment epithelium.

doi:10.3109/02713689508998495

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Article

An improved method of isolating fetal human retinal pigment epithelium.

Current eye research - 1 Aug 1995

Castillo B V, Little C W, del Cerro C, del Cerro M

Abstract excerpt

The purpose of this study was to develop an improved method of isolating fetal human retinal pigment epithelium (RPE) for tissue culture or transplantation. Fetal human eyes ranging from 8 to 20 wks of gestation were collected and stored in Optisol solution. Under a dissecting microscope, an inci...

Topics

Cell Separation
Cell Survival
Cells, Cultured
Coloring Agents
Culture Media
Endopeptidases
Fetus
Fluorescent Antibody Technique
Gestational Age
Humans
Keratins
Phenotype
Pigment Epithelium of Eye
Trypan Blue

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