Article
Mutants in disulfide bond formation that disrupt flagellar assembly in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1993
Dailey F E, Berg H C
Abstract excerpt
We report the isolation and characterization of Escherichia coli mutants (dsbB) that fail to assemble functional flagella unless cystine is present. Flagellar basal bodies obtained from these mutants are missing the L and P rings. This defect in assembly appears to result from an inability to form a disulfide bond in the P-ring protein (FlgI). Cystine suppresses this defect in dsbB strains. We also show that dsbA...
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