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Article

Analysis of the Bradyrhizobium japonicum hemH gene and its expression in Escherichia coli.

Applied and environmental microbiology - 1 Aug 1993

Frustaci J M, O'Brian M R

Abstract excerpt

Complementation analysis showed that the Bradyrhizobium japonicum hemH gene was both necessary and sufficient to rescue mutant strains I110ek4 and I110bk2 in trans with respect to hemin auxotrophy, protoporphyrin accumulation, and the deficiency in ferrochelatase activity. The B. japonicum hemH gene was expressed in an Escherichia coli T7 expression system and yielded a 39-kDa protein, which was consistent with...

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Identifiers

PMID: 8368826
DOI: 10.1128/aem.59.8.2347-2351.1993
PMCID: PMC182289

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Source receipt: pubmed, retrieved 7 Sept 2026.

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Analysis of the Bradyrhizobium japonicum hemH gene and its expression in Escherichia coli.PMID 8368826Read ↗

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