Article
Dimerization of Thermomonospora fusca beta-1,4-endoglucanase E2.
Biochemistry - 17 Aug 1993
McGinnis K, Kroupis C, Wilson D B
Abstract excerpt
Unboiled Thermomonospora fusca endoglucanase E2 electrophoresed on SDS-polyacrylamide gels migrated in the range of 80-90 kDa, but when boiled it migrated in the 40-42-kDa range. Sedimentation equilibrium centrifugation as well as chemical cross-linking experiments confirmed that E2 is a dimer. The dimer was reversibly dissociated at low pH. The E2 dimer was stable up to 70 degrees C, but began to dissociate at...
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