Article
Cytochrome b562 variants: a library for examining redox potential evolution.
Biochemistry - 23 May 2000
Springs S L, Bass S E, McLendon G L
Abstract excerpt
A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through mutation at two positions is examined within a fixed structural motif. Random mutation of Phe61 and...
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