Article
Two-step polymerase chain reaction for diagnosis of scrub typhus and identification of antigenic variants of Rickettsia tsutsugamushi.
The Journal of veterinary medical science - 1 Oct 1993
Kawamori F, Akiyama M, Sugieda M, Kanda T, Akahane S, Yamamoto S, Ohashi N, Tamura A
Abstract excerpt
Two-step polymerase chain reaction (nested PCR) method was examined for the diagnosis of scrub typhus. Primers were derived from the type-specific antigen (TSA) gene DNA sequences of Rickettsia tsutsugamushi, Gilliam strain. These primers served to produce rickettsia-specific products in the ampl...
Topics
- Animals
- Antigens, Bacterial
- Base Sequence
- DNA Primers
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Humans
- Mice
- Mites
- Molecular Sequence Data
- Orientia tsutsugamushi