Article
Immunochemical studies of ferrochelatase protein: characterization of the normal and mutant protein in bovine and human protoporphyria.
American journal of human genetics - 1 Jan 1991
Straka J G, Hill H D, Krikava J M, Kools A M, Bloomer J R
Abstract excerpt
Protoporphyria is a hereditary disorder characterized by a marked decrease in the activity of ferrochelatase, the terminal enzyme in the heme biosynthetic pathway. We have prepared specific polyvalent antibodies against bovine ferrochelatase in rabbits. The specificity of the antibody preparation against ferrochelatase was demonstrated by western blot analysis and immunoprecipitation of ferrochelatase activity....
Topics
- Animals
- Blotting, Western
- Cattle
- Enzyme-Linked Immunosorbent Assay
- Ferrochelatase
- Humans
- Liver Diseases
- Microsomes, Liver
- Mutation
- Porphyrias
- Protoporphyria, Erythropoietic