Article
Identification of an allelic variant of isoform MLC1-V/sB (human myosin light chain).
Biochemical genetics - 1 Jun 1993
Laptev A V, Shishkin S S, Kovalyov L I, Galyuk M A, Musolyamov A Kh, Egorov T A
Abstract excerpt
A study of 250 specimens of human myocardium by two-dimensional gel electrophoresis revealed an allelic variant of isoform MLC1-V/sB, which was identified by immunoblotting with monoclonal antibody against MLC1-V/sB and peptide mapping after in situ tryptic digestion of electroblotted proteins. The substitution Asn-144 for His-144 was found in this new allelic variant of MLC1-V/sB.
Topics
- Alleles
- Amino Acid Sequence
- Chromatography, High Pressure Liquid
- Electrophoresis, Gel, Two-Dimensional
- Genetic Variation
- Humans
- Molecular Sequence Data
- Myocardium
- Myosins