Article
Polymerase chain reaction and direct sequencing of Neisseria gonorrhoeae protein IB gene: partial nucleotide and amino acid sequence analysis of strains S4, S11, S48 (serovar IB4) and S34 (serovar IB5).
Medical microbiology and immunology - 1 Jul 1993
Lau Q C, Chow V T, Poh C L
Abstract excerpt
A pair of primers were designed for the polymerase chain reaction (PCR) to amplify a 341-base pair fragment of the gene encoding the outer membrane protein IB (PIB) of Neisseria gonorrhoeae. This PCR technique is specific and sensitive, being able to detect gonococcal strains belonging to ten dif...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA, Bacterial
- Electrophoresis, Agar Gel
- Humans
- Molecular Sequence Data
- Neisseria gonorrhoeae
- Phenotype
- Polymerase Chain Reaction
- Porins
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid