Expression of the Neisseria gonorrhoeae major outer membrane protein PI in Escherichia coli

Abstract excerpt

To actively express an outer membrane protein, protein I (PI), from different strains of Neisseria gonorrhoeae in E. coli, PI gene fragments from two reference strains and four clinical isolates of Neisseria gonorrhoeae were obtained with PCR amplification. They were cloned into the PCR cloning vector pBS-T to form pBS-T-PI and sequenced. Subsequently, they were cloned into an expression vector pET-30b (+) to gene...

Topics

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• Amino Acid Sequence
• Anti-Bacterial Agents
• DNA, Bacterial
• DNA Mutational Analysis
• Drug Resistance, Bacterial
• Mutation
• Neisseria gonorrhoeae
• Polymerase Chain Reaction

Identifiers and source

Literature Corpus work

0aa2622b-a19f-5dfa-92fa-1524cfd39aa2

DOI

10.1007/s11274-005-9092-2