Article
Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon.
Journal of bacteriology - 1 Nov 1993
Steed P M, Wanner B L
Abstract excerpt
The phosphate regulon is negatively regulated by the PstSCAB transporter and PhoU protein by a mechanism that may involve protein-protein interaction(s) between them and the Pi sensor protein, PhoR. In order to study such presumed interaction(s), mutants with defined deletions of the pstSCAB-phoU...
Topics
- Alleles
- Bacterial Proteins
- Bacteriophage M13
- Escherichia coli
- Escherichia coli Proteins
- Gene Deletion
- Genes, Bacterial
- Genotype
- Membrane Proteins
- Membrane Transport Proteins
- Mutagenesis
- Mutagenesis, Site-Directed
- Operon
- Phosphates
- Plasmids
- Regulon
- Restriction Mapping
- Transcription Factors