Comparison of pyruvate kinase variants from rat liver and Morris hepatoma 7777, obtained by an affinity chromatography on blue sepharose CL-6B.

Article

Comparison of pyruvate kinase variants from rat liver and Morris hepatoma 7777, obtained by an affinity chromatography on blue sepharose CL-6B.

Acta biochimica Polonica - 1 Jan 1993

Ignacak J, Gumińska M

Abstract excerpt

Fractions A (salted out by ammonium sulphate between 21-30% saturation), and fractions B (salted out between 51-70% saturation) of pyruvate kinase (EC 2.7.1.40.) corresponding respectively to pyruvate kinase types L and M2 from rat liver and Morris hepatoma 7777 were purified by an affinity chrom...

Topics

Animals
Chromatography, Affinity
Cysteine
Cytosol
Genetic Variation
Isoenzymes
Kinetics
Liver
Liver Neoplasms, Experimental
Pyruvate Kinase
Rats
Sepharose

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