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Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay

1 Oct 1993

Abstract excerpt

The comet assay, a sensitive method of measuring DNA strand breaks in individual cells, is also capable of identifying apoptotic cells which contain highly fragmented DNA. This method requires embedding cells in agarose, lysing cells to remove proteins, and providing a brief exposure to an electric field to allow broken pieces of DNA to migrate. TK6 human B lymphoblast cells undergo fragmentation which is...

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PMID: 8210329
DOI: 10.2307/3578650

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