Article
Expression in Escherichia coli of a TetK determinant from Staphylococcus aureus.
Biological & pharmaceutical bulletin - 1 Feb 1994
Noguchi N, Emura A, Sasatsu M, Kono M
Abstract excerpt
The expression in Escherichia coli of a tet(K) gene, which originated in Staphylococcus aureus, was studied. The minimum inhibitory concentration (MIC) of tetracycline (TC) for E. coli cells that carried the tet(K) gene was only slightly higher than that for the recipient cells. This result indicated that the level of expression of the tet(K) gene in E. coli was very low. Insertion of a lac promoter into the...
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