Article
Phenotype and biological activity of neonatal equine chondrocytes cultured in a three-dimensional fibrin matrix.
American journal of veterinary research - 1 Mar 1994
Hendrickson D A, Nixon A J, Erb H N, Lust G
Abstract excerpt
Equine neonatal chondrocytes were cultured in three-dimensional fibrin matrices under conditions of immediate implantation or implantation following monolayer culture for 6 days, and 3 cell concentrations (1 x 10(5), 1 x 10(6), and 5 x 10(6) chondrocytes/cm3). Equine fibrinogen was collected by cryoprecipitation and polymerized by use of activated bovine thrombin. The fibrin implants were harvested and analyzed...
Topics
- Animals
- Animals, Newborn
- Cartilage, Articular
- Cell Differentiation
- Cells, Cultured
- Chondroitin Sulfates
- Culture Techniques
- Fibrin
- Glycosaminoglycans
- Horses
- Microscopy, Phase-Contrast
