Article
A mutation blocking the formation of membrane or periplasmic endogenous and exogenous c-type cytochromes in Escherichia coli permits the cytoplasmic formation of Hydrogenobacter thermophilus holo cytochrome c552.
FEBS letters - 16 May 1994
Sambongi Y, Crooke H, Cole J A, Ferguson S J
Abstract excerpt
A mutant of Escherichia coli, JCB606, shown to be pleiotropically deficient in the formation of endogenous membrane and periplasmic c-type cytochromes, synthesised the apo form of the exogenous cytochrome c550 from Paracoccus denitrificans, but not the holo form. In contrast, a cytoplasmically located holo form of Hydrogenobacter thermophilus cytochrome c552 was found in E. coli JCB606. These findings support the...
Topics
- Cytochrome c Group
- Cytoplasm
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gram-Negative Chemolithotrophic Bacteria
- Immunoblotting
- Molecular Weight
- Mutation
- Paracoccus denitrificans
- Plasmids