Article
Fast, simple and highly sensitive double‐rounded polymerase chain reaction assay to detect medically relevant fungi in dermatological specimens
1 Jun 2000
Abstract excerpt
BACKGROUND: Early detection of fungal infection is essential for beginning of prompt and specific therapy. In this study we describe a rapid and sensitive procedure to detect, by polymerase chain reaction (PCR) assays, a wide range of medically important opportunistic and pathogenic fungi in dermal specimens from dermatomycoses-affected patients. MATERIALS AND METHODS: Three primer pairs, amplifying fragments of...
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