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Article

Cloning of thienamycin biosynthetase genes from Streptomyces cattleya.

Chinese journal of biotechnology - 1 Jan 1993

Li R, Wang Y, Zeng Y

Abstract excerpt

A mutant Y3 blocked in the thienamycin biosynthetic pathway was obtained from thienamycin producing strain Streptomyces cattleya by NTG treatment. Preliminary cloning system has been established on the basis of studies on the conditions for protoplast formation, regeneration, as well as DNA transformation for Y3 mutant strain. The shotgun cloning was carried out from S. cattleya using pIJ680 as a vector and the...

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PMID: 8155834

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