Article
Isolation and characterization of mutants defective in production of laccase in Neurospora crassa.
Molecular & general genetics : MGG - 1 Aug 1993
Zamma A, Tamaru H, Harashima T, Inoue H
Abstract excerpt
A protein synthesis inhibitor, cycloheximide, induces excretion of laccase in Neurospora crassa. The lah-1 mutation results in excretion of a large amount of laccase even in the absence of cycloheximide. Ten mutations were induced that suppress derepressed excretion of laccase in the lah-1 mutant. Of these, seven second-site mutations were found to confer a laccase-noninducible phenotype, and were classified into...
Topics
- Blotting, Northern
- Enzyme Induction
- Gene Expression Regulation, Fungal
- Genetic Complementation Test
- Genetic Linkage
- Laccase
- Mutation
- Neurospora crassa
- Oxidoreductases
- Phenotype
- Polymorphism, Restriction Fragment Length