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Article

A PCR strategy that differentiates two alleles for a single base insertion within a T repeat.

BioTechniques - 1 Jan 1993

Lungu O, Silverstein S

Abstract excerpt

PCR amplification with modified primers, in combination with BcgI restriction fragment length polymorphism analysis, allowed us to differentiate a string of 7 Ts present in exon 7 of the 21-hydroxylase gene of man from the 8 Ts present in exon 7 of its pseudogene. This approach can substitute for...

Topics

Alleles
Base Sequence
Biotechnology
DNA
DNA Mutational Analysis
Exons
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Pseudogenes
Repetitive Sequences, Nucleic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Steroid 21-Hydroxylase

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Identifiers

PMID: 8093837

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8093837-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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