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Article

A PCR strategy that differentiates two alleles for a single base insertion within a T repeat.

BioTechniques - 1 Jan 1993

Lungu O, Silverstein S

Abstract excerpt

PCR amplification with modified primers, in combination with BcgI restriction fragment length polymorphism analysis, allowed us to differentiate a string of 7 Ts present in exon 7 of the 21-hydroxylase gene of man from the 8 Ts present in exon 7 of its pseudogene. This approach can substitute for hybridization with oligonucleotides, or for sequence analysis, when an allele containing a multiple repeated...

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PMID: 8093837

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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