Article
Tyr-341 of the beta subunit is a major Km-determining residue of TF1-ATPase: parallel effect of its mutations on Kd(ATP) of the beta subunit and on Km(ATP) of the alpha 3 beta 3 gamma complex.
Journal of biochemistry - 1 Apr 1994
Odaka M, Kaibara C, Amano T, Matsui T, Muneyuki E, Ogasahara K, Yutani K, Yoshida M
Abstract excerpt
Residue Tyr-341 of the F1-ATPase beta subunit from a thermophilic Bacillus strain, PS3, was mutagenized to leucine, cysteine or alanine. Each of the mutated beta subunits was isolated and its affinity for ATP-Mg was examined by means of difference circular dichroism and differential titration cal...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Calorimetry
- Catalysis
- Circular Dichroism
- Cysteine
- Kinetics
- Molecular Sequence Data
- Mutation
- Peptide Fragments
- Proton-Translocating ATPases
- Thermodynamics