Article
Reversal of clavulanate resistance conferred by a Ser-244 mutant of TEM-1 beta-lactamase as a result of a second mutation (Arg to Ser at position 164) that enhances activity against ceftazidime.
Antimicrobial agents and chemotherapy - 1 May 1994
Imtiaz U, Manavathu E K, Mobashery S, Lerner S A
Abstract excerpt
The mutation of Arg-244 to Ser (Arg-244-->Ser mutation) in the TEM-1 beta-lactamase has been shown to produce resistance to inactivation by clavulanate in the mutant enzyme and resistance to ampicillin plus clavulanate in a strain of Escherichia coli producing this enzyme. The Arg-164-->Ser mutat...
Topics
- Anti-Bacterial Agents
- Ceftazidime
- Circular Dichroism
- Clavulanic Acid
- Clavulanic Acids
- Drug Resistance, Microbial
- Escherichia coli
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- beta-Lactamase Inhibitors
- beta-Lactamases