Article
Non-recombinant background in gene targeting: illegitimate recombination between a hpt gene and a defective 5' deleted nptII gene can restore a Kmr phenotype in tobacco.
Plant molecular biology - 1 Jul 1994
de Groot M J, Offringa R, Groet J, Does M P, Hooykaas P J, van den Elzen P J
Abstract excerpt
Previously we have demonstrated gene targeting in plants after Agrobacterium-mediated transformation. In these initial experiments a transgenic tobacco line 104 containing a T-DNA insertion with a defective neomycin phosphotransferase (nptII) gene was transformed with a repair construct containin...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA
- DNA Primers
- DNA, Bacterial
- Electroporation
- Kanamycin Kinase
- Molecular Sequence Data
- Phenotype
- Phosphotransferases (Alcohol Group Acceptor)
- Plants, Genetically Modified
- Plants, Toxic