Article
Several small GTP-binding proteins are strongly down-regulated in simian virus 40 (SV40) transformed human keratinocytes and may be required for the maintenance of the normal phenotype.
Electrophoresis - 1 Jan 2000
Gromov P S, Celis J E
Abstract excerpt
High resolution two-dimensional (2-D) gel electrophoresis in combination with the blot overlay nucleotide binding assay was used to reveal low molecular weight GTP-binding proteins expressed by primary cultured, normal human keratinocytes. Forty one small GTP-binding proteins (30 isoelectric focu...
Topics
- Autoradiography
- Cell Differentiation
- Cell Transformation, Neoplastic
- Cells, Cultured
- Electrophoresis, Polyacrylamide Gel
- GTP-Binding Proteins
- Guanosine Triphosphate
- Humans
- Isoelectric Focusing
- Keratinocytes
- Methionine
- Molecular Weight
- Phenotype
- Phosphorus Radioisotopes
- Simian virus 40
- Sulfur Radioisotopes