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Multiplex strand displacement amplification (SDA) and detection of DNA sequences from<i>Mycobacterium tuberculosis</i>and other mycobacteria

1 Jan 1994

Abstract excerpt

Strand Displacement Amplification (SDA) is an isothermal, in vitro method of amplifying a DNA target sequence prior to detection [Walker et al (1992) Nucleic Acids Res., 20, 1691-1693]. Here we describe a multiplex form of SDA that allows two target sequences and an internal amplification control to be co-amplified by a single pair of primers after common priming sequences are spontaneously appended to the ends...

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PMID: 8041630
DOI: 10.1093/nar/22.13.2670

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