PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes.

doi:10.1128/jcm.35.10.2689-2691.1997

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes.

Journal of clinical microbiology - 1 Oct 1997

Saunders N A, Hallas G, Gaworzewska E T, Metherell L, Efstratiou A, Hookey J V, George R C

Abstract excerpt

A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types th...

Topics

Antigens, Bacterial
Bacterial Outer Membrane Proteins
Bacterial Proteins
Biological Specimen Banks
Carrier Proteins
Enzyme-Linked Immunosorbent Assay
Genotype
Molecular Sequence Data
Polymerase Chain Reaction
Reference Standards
Sequence Analysis, DNA
Serotyping
Streptococcus pyogenes

Source

Open in PubMed Open full text

Identifiers

PMID: 9316938
DOI: 10.1128/jcm.35.10.2689-2691.1997
PMCID: PMC230041

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9316938-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics