Article
PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes.
Journal of clinical microbiology - 1 Oct 1997
Saunders N A, Hallas G, Gaworzewska E T, Metherell L, Efstratiou A, Hookey J V, George R C
Abstract excerpt
A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that were not included in the panel of capture probes were emm genotyped by sequencing.
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