Article
Gene expression in tobacco low-nicotine mutants.
The Plant cell - 1 May 1994
Hibi N, Higashiguchi S, Hashimoto T, Yamada Y
Abstract excerpt
Two nuclear genes, Nic1 and Nic2, regulate nicotine levels in tobacco. nic1 and nic2 are semidominant mutations in Burley 21 that reduce leaf nicotine levels and the activities of multiple enzymes in the nicotine pathway and simultaneously increase polyamine levels in cultured roots. Cultured roots homozygous for both mutations were used to isolate two cDNAs by subtraction hybridization; the transcript levels of...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cells, Cultured
- Escherichia coli
- Gene Expression
- Genes, Plant
- Genotype
- Homozygote
- Humans
- Mice
- Molecular Sequence Data