Article
Catabolite inactivation of heterologous fructose-1,6-bisphosphatases and fructose-1,6-bisphosphatase-beta-galactosidase fusion proteins in Saccharomyces cerevisiae.
European journal of biochemistry - 15 Jun 1994
Gamo F J, Navas M A, Blazquez M A, Gancedo C, Gancedo J M
Abstract excerpt
Fructose-1,6-bisphosphatase (FruP2ase) from Saccharomyces cerevisiae is rapidly inactivated upon addition of glucose to a culture growing on non-sugar carbon sources. Under the same conditions the FruP2ases from Schizosaccharomyces pombe or Escherichia coli expressed in S. cerevisiae were not aff...
Topics
- Amino Acid Sequence
- Base Sequence
- Culture Media
- Fructose-Bisphosphatase
- Glucose
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Ubiquitins
- Vacuoles
- beta-Galactosidase