Article
Subcellular localization, abundance and stability of chitin synthetases 1 and 2 from Saccharomyces cerevisiae.
Microbiology (Reading, England) - 1 Sept 1994
Leal-Morales C A, Bracker C E, Bartnicki-Garcia S
Abstract excerpt
The existence of more than one chitin synthetase in fungal cells poses the question of whether these enzymes have similar or different localization. The subcellular distribution of chitin synthetases 1 and 2 (Chs1 and Chs2) was determined in cell-free extracts of Saccharomyces cerevisiae fraction...
Topics
- Centrifugation, Density Gradient
- Chitin Synthase
- Cobalt
- Culture Media
- Enzyme Activation
- Enzyme Stability
- Glucosyltransferases
- Hydrostatic Pressure
- Membrane Proteins
- Microscopy, Electron
- Mutation
- Saccharomyces cerevisiae
- Schizosaccharomyces pombe Proteins
- Subcellular Fractions
- Trypsin