Article
A novel determinant (comA) essential for natural transformation competence in Neisseria gonorrhoeae and the effect of a comA defect on pilin variation.
Molecular microbiology - 1 Nov 1993
Facius D, Meyer T F
Abstract excerpt
A novel genetic determinant (comA) has been identified and found to be required for the transformation of piliated Neisseria gonorrhoeae. Mutants in comA of strain MS11 grow normally and are DNA-uptake proficient but blocked in the translocation of DNA into the cytoplasm. Here we show by site-specific mutagenesis and genetic complementation that only one of two open reading frames identified in comA is essential...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- DNA, Bacterial
- DNA-Binding Proteins
- Fimbriae Proteins
- Fimbriae, Bacterial