Direct sequencing and bidirectional allele specific polymerase chain reaction of the bovine beta-casein B variant.

Abstract excerpt

We have used the polymerase chain reaction (PCR) to amplify exon VII of the bovine beta-casein gene. The mutations responsible for the B variant were identified by direct sequencing of the amplification products. A bidirectional allele-specific PCR method (BAS-PCR) has been developed using oligonucleotides overlapping the mutation site at their 3' ends. This new procedure allows a rapid and reliable...

Topics

• Alleles
• Animals
• Base Sequence
• Caseins
• Cattle
• DNA
• DNA Mutational Analysis
• Evaluation Studies as Topic
• Exons
• Female
• Genetic Variation
• Molecular Sequence Data