Article
In vitro identification of retinal ganglion cells in culture without the need of dye labeling.
Journal of neuroscience methods - 1 Mar 1994
Guenther E, Schmid S, Grantyn R, Zrenner E
Abstract excerpt
We here describe a method for the identification of a distinct neuronal phenotype at all stages of development in culture without the need of any staining procedure. Based purely on a size criterion we can rapidly select vital retinal ganglion cells (RGCs) for further studies out of a mixed cultu...
Topics
- Animals
- Antigens, Surface
- Biomarkers
- Cell Size
- Cells, Cultured
- Membrane Glycoproteins
- Nerve Tissue Proteins
- Phenotype
- Rats
- Rats, Inbred BN
- Retina
- Retinal Ganglion Cells
- Thy-1 Antigens
