Article
[Polymerase chain reaction, cold probes and clinical diagnosis].
Sante (Montrouge, France) - 1 Jan 2000
Haras D, Amoros J P
Abstract excerpt
Until 1985 the only way to study a gene was to clone it. Henceforth, the polymerase chain reaction (PCR) is an alternative method for synthesizing millions of copies of a specific DNA sequence. Without the development of non radioactive probes, these technologies would have been reserved for rese...
Topics
- Base Sequence
- DNA Probes
- DNA-Directed DNA Polymerase
- Genetic Testing
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Amplification Techniques
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Reproducibility of Results
- Sensitivity and Specificity
- Taq Polymerase
- Temperature