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Article

Nonrepresentative PCR amplification of variable gene sequences in clinical specimens containing dilute, complex mixtures of microorganisms.

Journal of clinical microbiology - 1 Feb 1994

Wright C J, Jerse A E, Cohen M S, Cannon J G, Seifert H S

Abstract excerpt

PCR amplification and DNA sequencing of the expression locus from Neisseria gonorrhoeae contained in urine sediments collected from experimentally infected human subjects produced two observations. First, different pilin sequences were obtained when separate aliquots of the same sample were amplified and sequenced. In contrast, the same pilin sequence was obtained when repeated amplifications were performed on...

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Identifiers

PMID: 7908674
DOI: 10.1128/jcm.32.2.464-468.1994
PMCID: PMC263055

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