Nonrepresentative PCR amplification of variable gene sequences in clinical specimens containing dilute, complex mixtures of microorganisms.

doi:10.1128/jcm.32.2.464-468.1994

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Article

Nonrepresentative PCR amplification of variable gene sequences in clinical specimens containing dilute, complex mixtures of microorganisms.

Journal of clinical microbiology - 1 Feb 1994

Wright C J, Jerse A E, Cohen M S, Cannon J G, Seifert H S

Abstract excerpt

PCR amplification and DNA sequencing of the expression locus from Neisseria gonorrhoeae contained in urine sediments collected from experimentally infected human subjects produced two observations. First, different pilin sequences were obtained when separate aliquots of the same sample were ampli...

Topics

Adolescent
Adult
Bacterial Proteins
Base Sequence
DNA, Bacterial
Fimbriae Proteins
Fimbriae, Bacterial
Genes, Bacterial
Genetic Variation
Gonorrhea
Humans
Male
Molecular Sequence Data
Neisseria gonorrhoeae
Polymerase Chain Reaction

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Identifiers

PMID: 7908674
DOI: 10.1128/jcm.32.2.464-468.1994
PMCID: PMC263055

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7908674-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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