Article
A side chain at position 48 of the human immunodeficiency virus type-1 protease flap provides an additional specificity determinant.
Virology - 10 Mar 1995
Moody M D, Pettit S C, Shao W, Everitt L, Loeb D D, Hutchison C A, Swanstrom R
Abstract excerpt
Substitution of glycine with glutamic acid at position 48 of the human immunodeficiency virus protease resulted in an enzyme with reduced activity on one of the protease processing sites in the viral Pol polyprotein precursor. Cleavage at this site was restored by a second-site substitution in th...
Topics
- Amino Acid Sequence
- Binding Sites
- Escherichia coli
- Gene Products, gag
- Gene Products, pol
- HIV Protease
- HIV-1
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Molecular Structure
- Mutagenesis, Site-Directed
- Oligopeptides
- Phenotype
- Plasmids
- Protein Processing, Post-Translational
- Substrate Specificity