Article
A research on DLA-DRB1 genotyping by PCR-RFLP. I. To select a appropriate oligonucleotide primer pair.
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao - 1 Jan 1994
He Y W, Ferencik S, Grosse-Wilde H
Abstract excerpt
In order to study the DLA (Dog Leucocyte Antigen) class II region we utilized the polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) method, which has been reported previously as an efficient and simple technique for accurate definition of the HLA class II alleles. To search for a appropriate primer pair a series of amplifications with 4 different primer pairs DLA-DR-SP/Stop,...
Topics
- Alleles
- Animals
- Base Sequence
- DNA Primers
- Dogs
- Genotype
- Histocompatibility Antigens
- Histocompatibility Antigens Class I
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length